Self limiting multiplexed assembly of li

This study investigates the self-limiting assembly of phospholipid membranes with diverse functionalities on large-area graphene sensor arrays, leveraging the capabilities of multi-pen dip-pen nano-lithography (DPN) to achieve simultaneous and controlled functionalization. The primary objective is to enhance the efficiency and selectivity of graphene in sensor applications by integrating bio-mimetic lipid membranes that can selectively recognize interacting molecular species without compromising the intrinsic electronic properties of the graphene. Methodologically, we applied multi-pen DPN to deposit lipid layers, exploiting the characteristics of graphene to confine lipid spreading, thus negating the need for probe scanning and reducing the writing time required. Our findings demonstrate that the lipids maintain their functional properties post-assembly and facilitate the development of multiplexed biosensors with enhanced selectivity. The results indicate significant advancements in the controlled immobilization of membranes on sensor surfaces, paving the way for more sophisticated bio-sensor designs capable of responding to various environmental stimuli.