Talaromyces pseudofuniculosus leaching mineralization coupled 2027 Minerals

Microbial methods offer environmentally friendly, low-energy strategies for enhanced mineral CO2 sequestration. Most studies focus on mineral leaching, and while coupling leaching with mineralization to achieve efficient carbon fixation remains limited. In this study, Talaromyces pseudofuniculosus . hzt-3 ′ was employed to promote wollastonite dissolution. Leaching conditions were optimized using single-factor experiments and response surface methodology (RSM), evaluating the effects of initial pH, inoculum size, pulp density, and temperature on Ca2+ and Si4+ leaching. Optimal parameters were initial pH 7.35, inoculum size 1.17 %, pulp density 0.4 %, and temperature 30 ◦C, achieving 88.79 % Ca2+ and 12.61 % Si4+ extraction. Residue analysis revealed reduced lattice spacing, more dispersed Ca2+ distribution, and that fungal-secreted organic acids lowered solution pH and complexed with Ca2+, promoting dissolution; hyphal mechanical action disrupted the mineral lattice, enhancing reactivity. Carbonation of the leachate under alkaline conditions (40 ◦C, CO2 flow rate 100 mL/min, stirring speed 500 rpm, NH3⋅H2O concentration 1.2 mol/L) reached 97.29 % Ca2+ mineralization efficiency within 5 min. This work establishes a fungal-enhanced wollastonite leaching and indirect mineralization strategy for CO2 sequestration, providing theoretical and experimental foundations for industrial.