In situ immobilization of heavy metals associated with uranium leach mines by bacterial sulfate reduction

Laboratory experiments with mixed populations of sulfate-reducing bacteria were shown to mediate the removal of milligrams/liter concentrations of uranium, selenium, arsenic, and vanadium from aqueous solution via reduction, precipitation, and adsorption. The objective of this study was to investigate the potential of in situ immobilization of heavy metals associated with uranium leach mines through enhanced microbial activity. Methodology included performing laboratory experiments with active sulfidogenic biomass to assess the effectiveness of injecting sulfate and a source of carbon to stimulate natural anaerobic microbial processes. Results indicated that such injections could significantly enhance microbial activity, leading to the successful immobilization of toxic metals in the surrounding environment of uranium leach mines. This research contributes to developing effective strategies for aquifer restoration following uranium mining by proposing a potential in situ remediation technique that combines biological processes with chemical amendments for improved heavy metal management.