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Optimizing Biogenic Cyanide Production Using Indigenous Cyanogenic Microorganisms for Bioleaching of Precious Metals

Anil Kumar, Alan Shemi

2025enbioleachingcyanogenic microorganismsprecious metalssustainable metallurgybioprocessing

Abstract

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The present study investigated and optimized biogenic cyanide (bio-CN) production by using mine indigenous bacterial strains with the aim of extracting precious metals from Upper Group Two (UG-2) platinum group metals (PGM) ore. Among all the bacterial strains tested, under optimized conditions, Pseudomonas brassicacearum produced the highest bio-CN (14.1 ± 1 mg/L) and was therefore used in subsequent experiments. The study examined glycine consumption during cyano-genesis, as well as the stability and speciation of bio-CN. The findings confirmed that while glycine was consumed during cyanogenesis, the produced bio-CN was not stable and decreased over time, transforming into different cyanide species. The dose–response analysis for P. brassicacearum showed EC50 of 1785 g/L and 107.9 g/L against untreated and pre-treated PGM concentrate, respectively, suggesting higher toxicity for the latter. Two-step bioleaching of the pre-treated PGM concentrate showed extractions of 75.7, 18.7, 9.4, and 0.3% for Au, Pd, Rh, and Pt, respectively, at 10 g/L pulp density. The findings on bio-cyanide stability and bio-CN speciation presented in this paper are novel contributions. This study has established a foundation for developing a complete bio-based approach to PGM bioprocessing from UG-2 ores.

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Cite This Work

@article{3c34766d-9c70-4e02-a2f4-4f9ea804e4a8,
  title={Optimizing Biogenic Cyanide Production Using Indigenous Cyanogenic Microorganisms for Bioleaching of Precious Metals},
  author={Anil Kumar and Alan Shemi},
  year={2025},
  language={en}
}
TY  - JOUR
TI  - Optimizing Biogenic Cyanide Production Using Indigenous Cyanogenic Microorganisms for Bioleaching of Precious Metals
AU  - Anil Kumar
AU  - Alan Shemi
PY  - 2025
LA  - en
ER  -

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