Electrospun biodegradable nanofibrous ma

In this study, electrospun biodegradable nanofibrous mats made of microbial polyester, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV), and its blends were investigated for tissue engineering applications. The objective was to evaluate the effects of various processing parameters on the fiber morphology and dimensions, particularly focusing on different PHBV concentrations (10% w/v, 15% w/v) and blends with lactide-based polymers. Methodology involved producing fibrous scaffolds from these materials and analyzing their characteristics, including fiber diameter and surface porosity. Results indicated that increasing the concentration of PHBV from 5% to 15% w/v led to a significant increase in fiber diameter from 284 ± 133 nm to 2200 ± 716 nm. Additionally, the use of blends resulted in fibers with fewer beads and more uniform diameters. Surface porosities varied among the different scaffolds, with the highest porosity observed in PHBV-PLLA. Furthermore, in vitro studies using human osteosarcoma cells demonstrated that the electrospun scaffolds effectively promoted cell growth, indicating their potential for applications in tissue engineering.