Jingsong Zhou, Jianxun Yi
To activate skeletal muscle contraction, action potentials must be sensed by dihydropyridine receptors (DHPRs) in the transverse (T) tubule, which signal the Ca2+ release channels (RyRs) in the sarcoplasmic reticulum (SR) to open. We demonstrate here an inhibitory effect of the T tubule on the production of sparks of Ca2+ release. Murine primary cultures were confocally imaged for Ca2+ detection and T tubule visualization. After 72 hrs of differentiation, T tubules extended from the periphery for less than 1/3 of the myotube radius. Spontaneous Ca2+ sparks were found away from the region of cells where tubules were found. Immunostaining showed RyR 1 and 3 isoforms in all areas, implying inhibition of both isoforms by a T tubule component. To test for a role of DHPRs in this inhibition, we imaged myotubes from dysgenic (mdg) mice, lacking DHPRs. These exhibited T tubule development and demonstrated variations in Ca2+ signaling patterns, reinforcing the proposed inhibitory role of T tubules with respect to Ca2+ release events.
@article{6b492f1a-a14c-4fbf-86c2-19917f1d6693,
title={A probable role of dihydropyridine receptors in repression of Ca2+ sparks, demonstrated in cultured mammalian muscle.},
author={Jingsong Zhou and Jianxun Yi},
year={2005},
language={en}
}TY - JOUR TI - A probable role of dihydropyridine receptors in repression of Ca2+ sparks, demonstrated in cultured mammalian muscle. AU - Jingsong Zhou AU - Jianxun Yi PY - 2005 LA - en ER -
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