Electrospun poly d l lactide co l lactid

Electrospinning is a well-established polymer processing technique that has garnered increased interest due to its versatility in biomedical applications. This study focuses on the development of novel electrospun polymeric scaffolds made from poly-D/L-lactide and poly-L-lactide in a 50:50 ratio, aiming to create a scaffold suitable for soft tissue engineering. Optimal electrospinning conditions were identified, and the resulting poly(D/L-lactide-co-L-lactide) electrospun hybrid microfibers exhibited a unique porous structure with a high surface area that closely mimicked the native extracellular matrix (ECM). To evaluate the cytocompatibility of these scaffolds, dermal fibroblasts were isolated from human skin biopsies and cultured on the scaffolds for five days. The results demonstrated that the fibroblasts adhered, migrated, and proliferated effectively on the created three-dimensional scaffolds. These findings indicate the potential of electrospun poly(D/L-lactide-co-L-lactide) scaffolds as substrates for regenerative medicine, particularly in the context of skin tissue engineering.