Background: Telomeric DNA at the ends of eukaryotic chromosomes protects genome integrity via telomerase activity, and in Saccharomyces cerevisiae, temperature-sensitive point mutations in cdc13 combined with deletion of exo1 (cdc13-1 exo1 mutants) generate survivors at elevated temperatures. The inheritance pattern of the thermal tolerance allele in heterozygous diploid genotypes was previously unknown. Materials and Methods: Temperature-sensitive haploid strains (cdc13-1 exo1:LEU) were mated with temperature-resistant strains (cdc13-1 exo1::HIS) to construct heterozygous diploids via crosses 1296×3182 (D) and 2561×3181 (C). Putative diploids were selected on media lacking leucine and histidine, then streaked on selective media (-L, -H) and YEPD and incubated at 37 °C to assess thermal tolerance and viable colony counts (cfu). Result: The heterozygous diploid D grew robustly at 37 °C, and its viable colony yield was significantly higher than that of the C diploids (P = 0.05). Conclusion: The growth of diploid D10 at 37 °C demonstrates that the temperature-resistant allele in cdc13-1 exo1 heterozygous diploids is a dominant phenotype, indicating a dominant mode of inheritance for thermal tolerance in this system.
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title={eversvd,+03 (7)},
author={Unknown},
year={2026},
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