Jiangning Wu, K. E. Taylor
The study investigates the effect of polyethylene glycol (PEG) as an additive on the horseradish peroxidase (HRP) catalyzed removal of phenol from wastewater across a concentration range of 1-10 mM. The primary objective is to optimize various reaction conditions including pH, HRP concentration, PEG concentration, and the molar ratio of hydrogen peroxide to phenol, aiming to maximize phenol removal efficiency while minimizing costs. The methodology includes experimental trials conducted at room temperature to assess the protective effect of PEG on HRP activity and its impact on reaction time. Findings reveal that the addition of PEG significantly enhances HRP effectiveness, reducing the amount needed for phenol solutions by 40-75 times compared to reactions without PEG. The optimum conditions determined are a pH of 8.0 and a hydrogen peroxide to phenol molar ratio of approximately 1.0. Reaction times for achieving at least 95% removal of phenol are recorded as 5 hours for 1 mM and 3 hours for 10 mM solutions. Additionally, while increasing HRP concentration notably decreases reaction time, changes in PEG concentration have little effect on this metric.
@article{f3b66e99-42a1-4c64-91f6-910659fe0e2d,
title={Optimization of the reaction conditions},
author={Jiangning Wu and K. E. Taylor},
year={2026},
language={en}
}TY - JOUR TI - Optimization of the reaction conditions AU - Jiangning Wu AU - K. E. Taylor PY - 2026 LA - en ER -
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